Help with animations

Hello! I recently started working with Aseprite. I want to create an animation for tModLoader (basically for making Terraria mods), but it requires the animation to be exported as a single .png file.

I understand that the frames need to be placed in one image, stacked vertically, but is it possible to actually preview and work on the animation in Aseprite the same way you would when using layers or a timeline?

Thanks in advance for any help!