Hi @khado, I’ve received your file. It’s possible to recover the data inside it, but it looks like you have reached an internal limit that Aseprite cannot handle: the number of layers. You have way too many layers and cels (41690 layers and 31117 cels).
Internally, in the .aseprite format, each frame has “chunks”, a layer is a chunk, a cel is a chunk, etc., and a frame cannot have more than 65535 chunks (so 41690+31117 is too much for the file format to handle). I’ve never thought about this case (how can you handle soo many layers 
).
I’ll try to send you back to your email your file splitted in several other files or frames.